PUBLICATION

• Engineered CRISPR-Cas9 nuclease with expanded targeting space Nishimasu H, Shi X, Ishiguro S, Gao L, Hirano S, Okazaki S, Noda T, Abudayyeh OO, Gootenberg JS, Mori H, Oura S, Holmes B, Tanaka M, Seki M, Hirano H, Aburatani H, Ishitani R, Ikawa M, Yachie N, Zhang F, Nureki O.Science. 2018 09 21;361(6408):1259-1262. doi: 10.1126/science.aas9129 Real-space and real-time dynamics of CRISPR-Cas9 visualized by high-speed atomic force microscopy Shibata M, Nishimasu H, Kodera N, Hirano S, Ando T, Uchihashi T, Nureki O.Nat Commun. 2017 11 10;8(1):1430. doi: 10.1038/s41467-017-01466-8 Crystal Structure of Silkworm PIWI-Clade Argonaute Siwi Bound to piRNA Matsumoto N, Nishimasu H, Sakakibara K, Nishida KM, Hirano T, Ishitani R, Siomi H, Siomi MC, Nureki O.Cell. 2016 Oct 06;167(2):484-497.e9. doi: 10.1016/j.cell.2016.09.002 Crystal Structure of Cpf1 in Complex with Guide RNA and Target DNA Yamano T, Nishimasu H, Zetsche B, Hirano H, Slaymaker IM, Li Y, Fedorova I, Nakane T, Makarova KS, Koonin EV, Ishitani R, Zhang F, Nureki O.Cell. 2016 May 05;165(4):949-62. doi: 10.1016/j.cell.2016.04.003 Structure and Engineering of Francisella novicida Cas9 Hirano H, Gootenberg JS, Horii T, Abudayyeh OO, Kimura M, Hsu PD, Nakane T, Ishitani R, Hatada I, Zhang F, Nishimasu H, Nureki O.Cell. 2016 Feb 25;164(5):950-61. doi: 10.1016/j.cell.2016.01.039 Crystal Structure of Staphylococcus aureus Cas9 Nishimasu H, Cong L, Yan WX, Ran FA, Zetsche B, Li Y, Kurabayashi A, Ishitani R, Zhang F, Nureki O.Cell. 2015 Aug 27;162(5):1113-26. doi: 10.1016/j.cell.2015.08.007 Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex Konermann S, Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib N, Gootenberg JS, Nishimasu H, Nureki O, Zhang F.Nature. 2015 Jan 29;517(7536):583-8. doi: 10.1038/nature14136 Crystal structure of Cas9 in complex with guide RNA and target DNA Nishimasu H, Ran FA, Hsu PD, Konermann S, Shehata SI, Dohmae N, Ishitani R, Zhang F, Nureki O.Cell. 2014 Feb 27;156(5):935-49. doi: 10.1016/j.cell.2014.02.001 Structure and function of Zucchini endoribonuclease in piRNA biogenesis Nishimasu H, Ishizu H, Saito K, Fukuhara S, Kamatani MK, Bonnefond L, Matsumoto N, Nishizawa T, Nakanaga K, Aoki J, Ishitani R, Siomi H, Siomi MC, Nureki O.Nature. 2012 Nov 08;491(7423):284-7. doi: 10.1038/nature11509 Structural basis for the bifunctionality of fructose-1,6-bisphosphate aldolase/phosphatase Fushinobu S, Nishimasu H, Hattori D, Song HJ, Wakagi T.Nature. 2011 Oct 09;478(7370):538-41. doi: 10.1038/nature10457