Generation and characterization of an antagonistic monoclonal antibody against an extracellular domain of mouse DP2 (CRTH2/GPR44) receptors for prostaglandin D2.

Nagata N, Iwanari H, Kumagai H, Kusano-Arai O, Ikeda Y, Aritake K, Hamakubo T, Urade Y. Generation and characterization of an antagonistic monoclonal antibody against an extracellular domain of mouse DP2 (CRTH2/GPR44) receptors for prostaglandin D2. PLoS One. 2017 Apr 10;12(4):e0175452. doi: 10.1371/journal.pone.0175452. eCollection 2017.

Clinical initiatives linking Japanese and Swedish healthcare resources on cancer studies utilizing Biobank Repositories.

Nishimura T, Kawamura T, Sugihara Y, Bando Y, Sakamoto S, Nomura M, Ikeda N, Ohira T, Fujimoto J, Tojo H, Hamakubo T, Kodama T, Andersson R, Fehniger TE, Kato H, Marko-Varga G. Clinical initiatives linking Japanese and Swedish healthcare resources on cancer studies utilizing Biobank Repositories. Clin Transl Med. 2014 Nov 22;3(1):61. doi: 10.1186/s40169-014-0038-x. eCollection 2014 Dec.

Laboratory-size three-dimensional x-ray microscope with Wolter type I mirror optics and an electron-impact water window x-ray source.

Ohsuka S, Ohba A, Onoda S, Nakamoto K, Nakano T, Miyoshi M, Soda K, Hamakubo T. Laboratory-size three-dimensional x-ray microscope with Wolter type I mirror optics and an electron-impact water window x-ray source. Rev Sci Instrum. 2014 Sep;85(9):093701. doi: 10.1063/1.4894468.

Protective effect of the long pentraxin PTX3 against histone-mediated endothelial cell cytotoxicity in sepsis.

Daigo K, Nakakido M, Ohashi R, Fukuda R, Matsubara K, Minami T, Yamaguchi N, Inoue K, Jiang S, Naito M, Tsumoto K, Hamakubo T. Protective effect of the long pentraxin PTX3 against histone-mediated endothelial cell cytotoxicity in sepsis. Sci Signal. 2014 Sep 16;7(343):ra88. doi: 10.1126/scisignal.2005522.

Generation of antibodies against membrane proteins.

Hamakubo T, Kusano-Arai O, Iwanari H. Generation of antibodies against membrane proteins. Biochim Biophys Acta. 2014 Nov;1844(11):1920-1924. doi: 10.1016/j.bbapap.2014.08.007. Epub 2014 Aug 15. Review.

Immunostaining of oxidized DJ-1 in human and mouse brains.

Saito Y, Miyasaka T, Hatsuta H, Takahashi-Niki K, Hayashi K, Mita Y, Kusano-Arai O, Iwanari H, Ariga H, Hamakubo T, Yoshida Y, Niki E, Murayama S, Ihara Y, Noguchi N. Immunostaining of oxidized DJ-1 in human and mouse brains. J Neuropathol Exp Neurol. 2014 Jul;73(7):714-28. doi: 10.1097/NEN.0000000000000087.

Conjugation-free, visual, and quantitative evaluation of inhibitors on protein tyrosine kinases and phosphatases with a luminescent Tb(III) complex.

Akiba H, Sumaoka J, Hamakubo T, Komiyama M. Conjugation-free, visual, and quantitative evaluation of inhibitors on protein tyrosine kinases and phosphatases with a luminescent Tb(III) complex. Anal Bioanal Chem. 2014 May;406(12):2957-64. doi: 10.1007/s00216-014-7707-x. Epub 2014 Mar 21.

Identification and characterization of the X-dimer of human P-cadherin: implications for homophilic cell adhesion.

Kudo S, Caaveiro JM, Goda S, Nagatoishi S, Ishii K, Matsuura T, Sudou Y, Kodama T, Hamakubo T, Tsumoto K. Identification and characterization of the X-dimer of human P-cadherin: implications for homophilic cell adhesion. Biochemistry. 2014 Mar 25;53(11):1742-52. doi: 10.1021/bi401341g. Epub 2014 Mar 10.

A mesodermal factor, T, specifies mouse germ cell fate by directly activating germline determinants.

Aramaki S, Hayashi K, Kurimoto K, Ohta H, Yabuta Y, Iwanari H, Mochizuki Y, Hamakubo T, Kato Y, Shirahige K, Saitou M. A mesodermal factor, T, specifies mouse germ cell fate by directly activating germline determinants. Dev Cell. 2013 Dec 9;27(5):516-29. doi: 10.1016/j.devcel.2013.11.001.

A baculoviral display system to assay viral entry.

Iida M, Yoshida T, Watari A, Yagi K, Hamakubo T, Kondoh M. A baculoviral display system to assay viral entry. Biol Pharm Bull. 2013;36(11):1867-9.

Identification of Wilms’ tumor 1-associating protein complex and its role in alternative splicing and the cell cycle.

Horiuchi K, Kawamura T, Iwanari H, Ohashi R, Naito M, Kodama T, Hamakubo T. Identification of Wilms’ tumor 1-associating protein complex and its role in alternative splicing and the cell cycle. J Biol Chem. 2013 Nov 15;288(46):33292-302. doi: 10.1074/jbc.M113.500397. Epub 2013 Oct 7.

G protein-dependent basal and evoked endothelial cell vWF secretion.

Rusu L, Andreeva A, Visintine DJ, Kim K, Vogel SM, Stojanovic-Terpo A, Chernaya O, Liu G, Bakhshi FR, Haberichter SL, Iwanari H, Kusano-Arai O, Suzuki N, Hamakubo T, Kozasa T, Cho J, Du X, Minshall RD. G protein-dependent basal and evoked endothelial cell vWF secretion. Blood. 2014 Jan 16;123(3):442-50. doi: 10.1182/blood-2013-03-489351. Epub 2013 Sep 30.

A novel monoclonal antibody against the C-terminal region of aquaporin-4.

Ramadhanti J, Huang P, Kusano-Arai O, Iwanari H, Sakihama T, Misu T, Fujihara K, Hamakubo T, Yasui M, Abe Y. A novel monoclonal antibody against the C-terminal region of aquaporin-4. Monoclon Antib Immunodiagn Immunother. 2013 Aug;32(4):270-6. doi: 10.1089/mab.2013.0007.

Modification of p115RhoGEF Ser(330) regulates its RhoGEF activity.

Chow CR, Suzuki N, Kawamura T, Hamakubo T, Kozasa T. Modification of p115RhoGEF Ser(330) regulates its RhoGEF activity. Cell Signal. 2013 Nov;25(11):2085-92. doi: 10.1016/j.cellsig.2013.06.012. Epub 2013 Jun 29.

Establishment of monoclonal antibodies against the extracellular domain that block binding of NMO-IgG to AQP4.

Miyazaki K, Abe Y, Iwanari H, Suzuki Y, Kikuchi T, Ito T, Kato J, Kusano-Arai O, Takahashi T, Nishiyama S, Ikeshima-Kataoka H, Tsuji S, Arimitsu T, Kato Y, Sakihama T, Toyama Y, Fujihara K, Hamakubo T, Yasui M. Establishment of monoclonal antibodies against the extracellular domain that block binding of NMO-IgG to AQP4. J Neuroimmunol. 2013 Jul 15;260(1-2):107-16. doi: 10.1016/j.jneuroim.2013.03.003.

Novel role of copper transport protein antioxidant-1 in neointimal formation after vascular injury.

Kohno T, Urao N, Ashino T, Sudhahar V, McKinney RD, Hamakubo T, Iwanari H, Ushio-Fukai M, Fukai T. Novel role of copper transport protein antioxidant-1 in neointimal formation after vascular injury. Arterioscler Thromb Vasc Biol. 2013 Apr;33(4):805-13. doi: 10.1161/ATVBAHA.112.300862. Epub 2013 Jan 24.