7/19, LSBM Monday Seminar Series #10, Rikiya Watanabe, Single-molecule analysis of bio-molecules and its application

Presenter: Rikiya Watanabe (Molecular Physiology Laboratory, Cluster for Pioneering Research, RIKEN)

Title: Single-molecule analysis of bio-molecules and its application
Date: July 19th (Mon.) 12PM~



Single-molecule analysis of bio-molecules offer key benefits over macroscopic assay methods, as they unlock the ability to quantify various biological phenomena. In particular, micron/nano technologies enable highly sensitive and quantitative bioassays at the single-molecule level, which are widely used for ultra-sensitive biomedical applications, e.g., digital PCR and digital ELISA. To expand the versatility, we recently developed a novel microsystem “SATORI” with emerging CRISPR-Cas technologies that allows an accurate and rapid detection of nucleic-acid at single-molecule level. SATORI with CRISPR-Cas13a detected single-stranded RNA targets (ssRNA) with maximal sensitivity of ~5 fM in <5 min, with high specificity, thereby enabling the rapid detection of viral ssRNA, i.e., SARS-CoV-2. In addition, SATORI with CRISPR-Cas12a enabled single-molecule detection of double-stranded DNA, which could be applied to the rapid detection of circulating tumor DNAs for cancer diagnostics. Collectively, we expect that SATORI will be one of the key technologies for future diagnostics of infectious diseases and disorders.

Figure 1. Our microsystems for single-molecule analysis of bio-molecules