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| Systems Biology and Medicine Database |
The Objective
| SBM-DB aims to investigate mainly Human disease and other mammalian's.
Analysis from comprehensive gene expression profiles may help to define out the causes of disease in molecular level. |
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Experimental Procedure
| Experimental procedures for GeneChip were performed according to the Affymetrix GeneChip Expression Analysis Technical Manual. Briefly, each 10 mg RNA are used to synthesize biotin-labeled cRNA, which was then hybridized to the high-density oligonucleotide array (GeneChip Human U133 array; Affymetrix, Santa Crara, CA). After washing, arrays were stained with streptavidin-phycoerythrin and analyzed by a Hewlett-Packard Scanner to collect image data. |
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Data Analysis
| GeneChip Analysis Suite software version 5.0 was used to calculate the Signal value, and Detection Call (called "Average Difference", "Absolute Call" in previous MAS4.0). |
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Probe Information
| Each Probeset have a GenBank Accession number, and we mapped with UniGene ClusterID. (Human Unigene Build #148, 1 April, 2002) |
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Term of Use
| 1. |
User should obey the basis of these database objectives.
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Data should not be used for commercial pursuits.
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Data should not be transferred without permission.
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Data using the part of database should not be published without contact to the researcher who is responsible for the experiment.
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| 5. |
We do not guarantee the accuracy and reliability of our data, and are not responsible for the consequences of the possible errors.
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Copyright
| The experiment data and all other information in this websites are protected by copyright laws.
On the occasion of the duplicate of this information, permission is required. |
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History
Update Edited
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2003/08/25 from HNT-DB to RefExA |
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Rename HNT-DB to RefExA due to increased samples.
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from HNT-DB (Human Normal Tissue Database)
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to RefExA (Reference database for gene Expression Analysis)
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Gene annotation is update to UniGene Hs build#161
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Add annotation of NetAffx(TM) of Analysis Center (Affymetrix inc.)
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Improvements of web interface. (graphical panel, bar graph, etc.) |
| 2003/07/08 Renewal in Human Normal Tissue DB (HNT DB) |
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40 normal tissues + 17 normal cells + 23 cancer cell lines
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Gene annotation is update to UniGene build #159
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| 2003/04/11 LSBM Website renewal. Interface of SBM-DB has been improved. |
| 2002/07/15 Update to 35 Human Normal Tissues, and interface is reformed |
| 2002/04/16 Open Human Normal 30 Tissue Database |
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Grant
| SBM-DB is supported from the Grant(s) following,
- Grant-in-Aid for Publication of Scientific Research Results, KAKENHI, JSPS (2003.4-2004.3)
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