LSBM uses four fundamental technologies essential for system biomedical science
Key Technologies for System Bio-Medicine
１．To comprehensively measure gene expression
We measure the expression level of RNA for almost all genes on human genes. In current planar DNA chips, about 50% of genes are low in sensitivity and difficult to measure. For these, we are developing a DNA chip that sterically densely arranges probes. For mouse, rat, drosophila and so on as well, we measure the expression of thousands to over 10,000 individuals. We are predicting and measuring all family genes from genome sequences for Zinc finger proteins such as human G protein-coupled receptor and nuclear receptor important as a chemical receptor.
２．Cluster expression data and predict linkage of biological information
We have already made database of measurement results of more than 1000 types of cells, organs and pathological conditions per 10,000 genes on average. At the same time, it is in the process of creating underlying data with 40 human organizations. We clustered these data and are proceeding with profiling of genes leading to specific functions. At the same time, we are developing a new information analysis technology system that clarifies mutual relationships between genetic information, protein information, and information exceeding animal species.
３．It also expresses the most difficult membrane protein with function [BV patent]
When making proteins from genes, even with the same amino acid sequence the protein can have astonishingly different structures. It also undergoes numerous modifications due to degrading enzymes, sugars and lipids. Among proteins, LSBM possesses a “BV patent” that expresses membrane proteins that are most difficult to express with functions using insect viruses. We advanced this patent and developed technology to create membrane protein complexes.
４．Systematically make antibody / Make antibody with function
Antibodies are the most useful tool for recognizing proteins. LSBM is preparing monoclonal antibodies for all 48 nuclear receptor receptors. We also succeeded in producing antibodies that inhibit receptor function on C protein 2, such as G protein coupled receptor. By systematic antibody preparation, it became possible to verify as wet science, starting with temporal and spatial localization of protein and complex formation.